CovidFranceV1, Main, Exploration, bibRecord, 000267

Viral RNA load as determined by cell culture as a management tool for discharge of SARS-CoV-2 patients from infectious disease wards.

Identifieur interne : 000267 ( Main/Exploration ); précédent : 000266; suivant : 000268

Viral RNA load as determined by cell culture as a management tool for discharge of SARS-CoV-2 patients from infectious disease wards.

Auteurs : Bernard La Scola [France] ; Marion Le Bideau [France] ; Julien Andreani [France] ; Van Thuan Hoang [France, Viêt Nam] ; Clio Grimaldier [France] ; Philippe Colson [France] ; Philippe Gautret [France] ; Didier Raoult [France]

Source :

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology [ 1435-4373 ] ; 2020.

RBID : pubmed:32342252

Descripteurs français

KwdFr :
- ARN viral (analyse), Animaux (MeSH), Betacoronavirus (isolement et purification), Betacoronavirus (physiologie), Cellules Vero (MeSH), Charge virale (MeSH), France (MeSH), Humains (MeSH), Infections à coronavirus (diagnostic), Infections à coronavirus (virologie), Manipulation d'échantillons (normes), Pandémies (MeSH), Pneumopathie virale (diagnostic), Pneumopathie virale (virologie), RT-PCR (MeSH), Sortie du patient (MeSH).
MESH :
- analyse : ARN viral.
- diagnostic : Infections à coronavirus, Pneumopathie virale.
- isolement et purification : Betacoronavirus.
- normes : Manipulation d'échantillons.
- physiologie : Betacoronavirus.
- virologie : Infections à coronavirus, Pneumopathie virale.
- Animaux, Cellules Vero, Charge virale, France, Humains, Pandémies, RT-PCR, Sortie du patient.
Wicri :
- geographic : France.

English descriptors

KwdEn :
- Animals (MeSH), Betacoronavirus (isolation & purification), Betacoronavirus (physiology), Chlorocebus aethiops (MeSH), Coronavirus Infections (diagnosis), Coronavirus Infections (virology), France (MeSH), Humans (MeSH), Pandemics (MeSH), Patient Discharge (MeSH), Pneumonia, Viral (diagnosis), Pneumonia, Viral (virology), RNA, Viral (analysis), Reverse Transcriptase Polymerase Chain Reaction (MeSH), Specimen Handling (standards), Vero Cells (MeSH), Viral Load (MeSH).
MESH :
- chemical , analysis : RNA, Viral.
- geographic : France.
- diagnosis : Coronavirus Infections, Pneumonia, Viral.
- isolation & purification : Betacoronavirus.
- physiology : Betacoronavirus.
- standards : Specimen Handling.
- virology : Coronavirus Infections, Pneumonia, Viral.
- Animals, Chlorocebus aethiops, Humans, Pandemics, Patient Discharge, Reverse Transcriptase Polymerase Chain Reaction, Vero Cells, Viral Load.

Abstract

In a preliminary clinical study, we observed that the combination of hydroxychloroquine and azithromycin was effective against SARS-CoV-2 by shortening the duration of viral load in Covid-19 patients. It is of paramount importance to define when a treated patient can be considered as no longer contagious. Correlation between successful isolation of virus in cell culture and Ct value of quantitative RT-PCR targeting E gene suggests that patients with Ct above 33-34 using our RT-PCR system are not contagious and thus can be discharged from hospital care or strict confinement for non-hospitalized patients.

DOI: 10.1007/s10096-020-03913-9
PubMed: 32342252
PubMed Central: PMC7185831

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 001560
to stream Main, to step Curation: 001560

Le document en format XML

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<series><title level="j">European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology</title>
<idno type="eISSN">1435-4373</idno>
<imprint><date when="2020" type="published">2020</date>
</imprint>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term>
<term>Betacoronavirus (isolation & purification)</term>
<term>Betacoronavirus (physiology)</term>
<term>Chlorocebus aethiops (MeSH)</term>
<term>Coronavirus Infections (diagnosis)</term>
<term>Coronavirus Infections (virology)</term>
<term>France (MeSH)</term>
<term>Humans (MeSH)</term>
<term>Pandemics (MeSH)</term>
<term>Patient Discharge (MeSH)</term>
<term>Pneumonia, Viral (diagnosis)</term>
<term>Pneumonia, Viral (virology)</term>
<term>RNA, Viral (analysis)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (MeSH)</term>
<term>Specimen Handling (standards)</term>
<term>Vero Cells (MeSH)</term>
<term>Viral Load (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (analyse)</term>
<term>Animaux (MeSH)</term>
<term>Betacoronavirus (isolement et purification)</term>
<term>Betacoronavirus (physiologie)</term>
<term>Cellules Vero (MeSH)</term>
<term>Charge virale (MeSH)</term>
<term>France (MeSH)</term>
<term>Humains (MeSH)</term>
<term>Infections à coronavirus (diagnostic)</term>
<term>Infections à coronavirus (virologie)</term>
<term>Manipulation d'échantillons (normes)</term>
<term>Pandémies (MeSH)</term>
<term>Pneumopathie virale (diagnostic)</term>
<term>Pneumopathie virale (virologie)</term>
<term>RT-PCR (MeSH)</term>
<term>Sortie du patient (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term>
</keywords>
<keywords scheme="MESH" type="geographic" xml:lang="en"><term>France</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN viral</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term>
<term>Pneumonia, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term>
<term>Pneumopathie virale</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Betacoronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Betacoronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="normes" xml:lang="fr"><term>Manipulation d'échantillons</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Betacoronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Betacoronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="standards" xml:lang="en"><term>Specimen Handling</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term>
<term>Pneumopathie virale</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term>
<term>Pneumonia, Viral</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Chlorocebus aethiops</term>
<term>Humans</term>
<term>Pandemics</term>
<term>Patient Discharge</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
<term>Vero Cells</term>
<term>Viral Load</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cellules Vero</term>
<term>Charge virale</term>
<term>France</term>
<term>Humains</term>
<term>Pandémies</term>
<term>RT-PCR</term>
<term>Sortie du patient</term>
</keywords>
<keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>France</term>
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<front><div type="abstract" xml:lang="en">In a preliminary clinical study, we observed that the combination of hydroxychloroquine and azithromycin was effective against SARS-CoV-2 by shortening the duration of viral load in Covid-19 patients. It is of paramount importance to define when a treated patient can be considered as no longer contagious. Correlation between successful isolation of virus in cell culture and Ct value of quantitative RT-PCR targeting E gene suggests that patients with Ct above 33-34 using our RT-PCR system are not contagious and thus can be discharged from hospital care or strict confinement for non-hospitalized patients.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">32342252</PMID>
<DateCompleted><Year>2020</Year>
<Month>05</Month>
<Day>18</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>09</Month>
<Day>12</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1435-4373</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>39</Volume>
<Issue>6</Issue>
<PubDate><Year>2020</Year>
<Month>Jun</Month>
</PubDate>
</JournalIssue>
<Title>European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology</Title>
<ISOAbbreviation>Eur. J. Clin. Microbiol. Infect. Dis.</ISOAbbreviation>
</Journal>
<ArticleTitle>Viral RNA load as determined by cell culture as a management tool for discharge of SARS-CoV-2 patients from infectious disease wards.</ArticleTitle>
<Pagination><MedlinePgn>1059-1061</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1007/s10096-020-03913-9</ELocationID>
<Abstract><AbstractText>In a preliminary clinical study, we observed that the combination of hydroxychloroquine and azithromycin was effective against SARS-CoV-2 by shortening the duration of viral load in Covid-19 patients. It is of paramount importance to define when a treated patient can be considered as no longer contagious. Correlation between successful isolation of virus in cell culture and Ct value of quantitative RT-PCR targeting E gene suggests that patients with Ct above 33-34 using our RT-PCR system are not contagious and thus can be discharged from hospital care or strict confinement for non-hospitalized patients.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>La Scola</LastName>
<ForeName>Bernard</ForeName>
<Initials>B</Initials>
<Identifier Source="ORCID">http://orcid.org/0000-0001-8006-7704</Identifier>
<AffiliationInfo><Affiliation>IHU-Méditerranée Infection, Marseille, France. bernard.la-scola@univ-amu.fr.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Aix Marseille Univ, IRD, APHM, MEPHI, Marseille, France. bernard.la-scola@univ-amu.fr.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Le Bideau</LastName>
<ForeName>Marion</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>IHU-Méditerranée Infection, Marseille, France.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Aix Marseille Univ, IRD, APHM, MEPHI, Marseille, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Andreani</LastName>
<ForeName>Julien</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>IHU-Méditerranée Infection, Marseille, France.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Aix Marseille Univ, IRD, APHM, MEPHI, Marseille, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hoang</LastName>
<ForeName>Van Thuan</ForeName>
<Initials>VT</Initials>
<AffiliationInfo><Affiliation>IHU-Méditerranée Infection, Marseille, France.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Aix Marseille Univ, IRD, AP-HM, SSA, VITROME, Marseille, France.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Thai Binh University of Medicine and Pharmacy, Thai Binh, Viet Nam.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Grimaldier</LastName>
<ForeName>Clio</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>IHU-Méditerranée Infection, Marseille, France.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Aix Marseille Univ, IRD, APHM, MEPHI, Marseille, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Colson</LastName>
<ForeName>Philippe</ForeName>
<Initials>P</Initials>
<AffiliationInfo><Affiliation>IHU-Méditerranée Infection, Marseille, France.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Aix Marseille Univ, IRD, APHM, MEPHI, Marseille, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Gautret</LastName>
<ForeName>Philippe</ForeName>
<Initials>P</Initials>
<AffiliationInfo><Affiliation>IHU-Méditerranée Infection, Marseille, France.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Aix Marseille Univ, IRD, AP-HM, SSA, VITROME, Marseille, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Raoult</LastName>
<ForeName>Didier</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>IHU-Méditerranée Infection, Marseille, France. didier.raoult@gmail.com.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Aix Marseille Univ, IRD, APHM, MEPHI, Marseille, France. didier.raoult@gmail.com.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2020</Year>
<Month>04</Month>
<Day>27</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>Germany</Country>
<MedlineTA>Eur J Clin Microbiol Infect Dis</MedlineTA>
<NlmUniqueID>8804297</NlmUniqueID>
<ISSNLinking>0934-9723</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance>
</Chemical>
</ChemicalList>
<SupplMeshList><SupplMeshName Type="Disease" UI="C000657245">COVID-19</SupplMeshName>
<SupplMeshName Type="Organism" UI="C000656484">severe acute respiratory syndrome coronavirus 2</SupplMeshName>
</SupplMeshList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000073640" MajorTopicYN="N">Betacoronavirus</DescriptorName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018352" MajorTopicYN="N">Coronavirus Infections</DescriptorName>
<QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005602" MajorTopicYN="N" Type="Geographic">France</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D058873" MajorTopicYN="N">Pandemics</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010351" MajorTopicYN="Y">Patient Discharge</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011024" MajorTopicYN="N">Pneumonia, Viral</DescriptorName>
<QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013048" MajorTopicYN="N">Specimen Handling</DescriptorName>
<QualifierName UI="Q000592" MajorTopicYN="N">standards</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014709" MajorTopicYN="N">Vero Cells</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019562" MajorTopicYN="Y">Viral Load</DescriptorName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Co-culture</Keyword>
<Keyword MajorTopicYN="N">Correlation</Keyword>
<Keyword MajorTopicYN="N">Covid-19</Keyword>
<Keyword MajorTopicYN="N">RT-PCR</Keyword>
<Keyword MajorTopicYN="N">SARS-CoV2</Keyword>
<Keyword MajorTopicYN="N">Viral load</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year>
<Month>03</Month>
<Day>31</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2020</Year>
<Month>04</Month>
<Day>21</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2020</Year>
<Month>4</Month>
<Day>29</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2020</Year>
<Month>5</Month>
<Day>19</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2020</Year>
<Month>4</Month>
<Day>29</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">32342252</ArticleId>
<ArticleId IdType="doi">10.1007/s10096-020-03913-9</ArticleId>
<ArticleId IdType="pii">10.1007/s10096-020-03913-9</ArticleId>
<ArticleId IdType="pmc">PMC7185831</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>France</li>
<li>Viêt Nam</li>
</country>
<region><li>Provence-Alpes-Côte d'Azur</li>
</region>
<settlement><li>Marseille</li>
</settlement>
</list>
<tree><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="La Scola, Bernard" sort="La Scola, Bernard" uniqKey="La Scola B" first="Bernard" last="La Scola">Bernard La Scola</name>
</region>
<name sortKey="Andreani, Julien" sort="Andreani, Julien" uniqKey="Andreani J" first="Julien" last="Andreani">Julien Andreani</name>
<name sortKey="Andreani, Julien" sort="Andreani, Julien" uniqKey="Andreani J" first="Julien" last="Andreani">Julien Andreani</name>
<name sortKey="Colson, Philippe" sort="Colson, Philippe" uniqKey="Colson P" first="Philippe" last="Colson">Philippe Colson</name>
<name sortKey="Colson, Philippe" sort="Colson, Philippe" uniqKey="Colson P" first="Philippe" last="Colson">Philippe Colson</name>
<name sortKey="Gautret, Philippe" sort="Gautret, Philippe" uniqKey="Gautret P" first="Philippe" last="Gautret">Philippe Gautret</name>
<name sortKey="Gautret, Philippe" sort="Gautret, Philippe" uniqKey="Gautret P" first="Philippe" last="Gautret">Philippe Gautret</name>
<name sortKey="Grimaldier, Clio" sort="Grimaldier, Clio" uniqKey="Grimaldier C" first="Clio" last="Grimaldier">Clio Grimaldier</name>
<name sortKey="Grimaldier, Clio" sort="Grimaldier, Clio" uniqKey="Grimaldier C" first="Clio" last="Grimaldier">Clio Grimaldier</name>
<name sortKey="Hoang, Van Thuan" sort="Hoang, Van Thuan" uniqKey="Hoang V" first="Van Thuan" last="Hoang">Van Thuan Hoang</name>
<name sortKey="Hoang, Van Thuan" sort="Hoang, Van Thuan" uniqKey="Hoang V" first="Van Thuan" last="Hoang">Van Thuan Hoang</name>
<name sortKey="La Scola, Bernard" sort="La Scola, Bernard" uniqKey="La Scola B" first="Bernard" last="La Scola">Bernard La Scola</name>
<name sortKey="Le Bideau, Marion" sort="Le Bideau, Marion" uniqKey="Le Bideau M" first="Marion" last="Le Bideau">Marion Le Bideau</name>
<name sortKey="Le Bideau, Marion" sort="Le Bideau, Marion" uniqKey="Le Bideau M" first="Marion" last="Le Bideau">Marion Le Bideau</name>
<name sortKey="Raoult, Didier" sort="Raoult, Didier" uniqKey="Raoult D" first="Didier" last="Raoult">Didier Raoult</name>
<name sortKey="Raoult, Didier" sort="Raoult, Didier" uniqKey="Raoult D" first="Didier" last="Raoult">Didier Raoult</name>
</country>
<country name="Viêt Nam"><noRegion><name sortKey="Hoang, Van Thuan" sort="Hoang, Van Thuan" uniqKey="Hoang V" first="Van Thuan" last="Hoang">Van Thuan Hoang</name>
</noRegion>
</country>
</tree>
</affiliations>
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Viral RNA load as determined by cell culture as a management tool for discharge of SARS-CoV-2 patients from infectious disease wards.

Viral RNA load as determined by cell culture as a management tool for discharge of SARS-CoV-2 patients from infectious disease wards.

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